Single residues in the LRR domain of the wheat PM3A immune receptor can control the strength and the spectrum of the immune response.
Identifieur interne : 000051 ( Main/Exploration ); précédent : 000050; suivant : 000052Single residues in the LRR domain of the wheat PM3A immune receptor can control the strength and the spectrum of the immune response.
Auteurs : Stefan Lindner [Suisse] ; Bettina Keller [Suisse] ; Simrat Pal Singh [Suisse] ; Zsuzsanna Hasenkamp [Suisse] ; Esther Jung [Suisse] ; Marion C. Müller [Suisse] ; Salim Bourras [Suisse, Suède] ; Beat Keller [Suisse]Source :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2020.
Abstract
The development of improved plant nucleotide-binding, leucine-rich repeat immune receptors (NLRs) has mostly been based on random mutagenesis or on structural information available for specific receptors complexed with the recognized pathogen effector. Here, we use a targeted mutagenesis approach based on natural diversity of the Pm3 powdery mildew resistance alleles present in different wheat genotypes. In order to understand the functional importance of the amino acid polymorphisms between the active immune receptor PM3A and the inactive ancestral variant PM3CS, we exchanged polymorphic regions and residues in the LRR domain of PM3A with the corresponding segments of PM3CS. These novel variants were functionally tested for recognition of the corresponding AVRPM3A2/F2 avirulence protein in Nicotiana benthamiana. We identified polymorphic residues in four regions of PM3A that enhance the immune response, but also residues that reduce it or result in complete loss of function. We found that the identified critical residues in PM3A modify its activation threshold towards different protein variants of AVRPM3A2/F2 . PM3A variants with a lowered threshold gave a stronger overall response and gained an extended recognition spectrum. One of these variant proteins with a single amino acid change was stably transformed into wheat where it conferred race-specific resistance to mildew. This is a proof of concept that improved PM3A variants with an enlarged recognition spectrum can be engineered based on natural diversity by exchanging single or multiple residues that modulate resistance function.
DOI: 10.1111/tpj.14917
PubMed: 32645755
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Single residues in the LRR domain of the wheat PM3A immune receptor can control the strength and the spectrum of the immune response.</title><author><name sortKey="Lindner, Stefan" sort="Lindner, Stefan" uniqKey="Lindner S" first="Stefan" last="Lindner">Stefan Lindner</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Keller, Bettina" sort="Keller, Bettina" uniqKey="Keller B" first="Bettina" last="Keller">Bettina Keller</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Pal Singh, Simrat" sort="Pal Singh, Simrat" uniqKey="Pal Singh S" first="Simrat" last="Pal Singh">Simrat Pal Singh</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Hasenkamp, Zsuzsanna" sort="Hasenkamp, Zsuzsanna" uniqKey="Hasenkamp Z" first="Zsuzsanna" last="Hasenkamp">Zsuzsanna Hasenkamp</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmacology and Toxicology, University of Zurich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Jung, Esther" sort="Jung, Esther" uniqKey="Jung E" first="Esther" last="Jung">Esther Jung</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Muller, Marion C" sort="Muller, Marion C" uniqKey="Muller M" first="Marion C" last="Müller">Marion C. Müller</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Bourras, Salim" sort="Bourras, Salim" uniqKey="Bourras S" first="Salim" last="Bourras">Salim Bourras</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32645755</idno><idno type="pmid">32645755</idno><idno type="doi">10.1111/tpj.14917</idno><idno type="wicri:Area/Main/Corpus">000197</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000197</idno><idno type="wicri:Area/Main/Curation">000197</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000197</idno><idno type="wicri:Area/Main/Exploration">000197</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Single residues in the LRR domain of the wheat PM3A immune receptor can control the strength and the spectrum of the immune response.</title><author><name sortKey="Lindner, Stefan" sort="Lindner, Stefan" uniqKey="Lindner S" first="Stefan" last="Lindner">Stefan Lindner</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Keller, Bettina" sort="Keller, Bettina" uniqKey="Keller B" first="Bettina" last="Keller">Bettina Keller</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Pal Singh, Simrat" sort="Pal Singh, Simrat" uniqKey="Pal Singh S" first="Simrat" last="Pal Singh">Simrat Pal Singh</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Hasenkamp, Zsuzsanna" sort="Hasenkamp, Zsuzsanna" uniqKey="Hasenkamp Z" first="Zsuzsanna" last="Hasenkamp">Zsuzsanna Hasenkamp</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmacology and Toxicology, University of Zurich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Jung, Esther" sort="Jung, Esther" uniqKey="Jung E" first="Esther" last="Jung">Esther Jung</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Muller, Marion C" sort="Muller, Marion C" uniqKey="Muller M" first="Marion C" last="Müller">Marion C. Müller</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author><author><name sortKey="Bourras, Salim" sort="Bourras, Salim" uniqKey="Bourras S" first="Salim" last="Bourras">Salim Bourras</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Plant and Microbial Biology, University of Zürich, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName><orgName type="university">Université de Zurich</orgName></affiliation></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="eISSN">1365-313X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The development of improved plant nucleotide-binding, leucine-rich repeat immune receptors (NLRs) has mostly been based on random mutagenesis or on structural information available for specific receptors complexed with the recognized pathogen effector. Here, we use a targeted mutagenesis approach based on natural diversity of the Pm3 powdery mildew resistance alleles present in different wheat genotypes. In order to understand the functional importance of the amino acid polymorphisms between the active immune receptor PM3A and the inactive ancestral variant PM3CS, we exchanged polymorphic regions and residues in the LRR domain of PM3A with the corresponding segments of PM3CS. These novel variants were functionally tested for recognition of the corresponding AVRPM3<sup>A2/F2</sup> avirulence protein in Nicotiana benthamiana. We identified polymorphic residues in four regions of PM3A that enhance the immune response, but also residues that reduce it or result in complete loss of function. We found that the identified critical residues in PM3A modify its activation threshold towards different protein variants of AVRPM3<sup>A2/F2</sup> . PM3A variants with a lowered threshold gave a stronger overall response and gained an extended recognition spectrum. One of these variant proteins with a single amino acid change was stably transformed into wheat where it conferred race-specific resistance to mildew. This is a proof of concept that improved PM3A variants with an enlarged recognition spectrum can be engineered based on natural diversity by exchanging single or multiple residues that modulate resistance function.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32645755</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-313X</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Jul</Month><Day>09</Day></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>Single residues in the LRR domain of the wheat PM3A immune receptor can control the strength and the spectrum of the immune response.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/tpj.14917</ELocationID><Abstract><AbstractText>The development of improved plant nucleotide-binding, leucine-rich repeat immune receptors (NLRs) has mostly been based on random mutagenesis or on structural information available for specific receptors complexed with the recognized pathogen effector. Here, we use a targeted mutagenesis approach based on natural diversity of the Pm3 powdery mildew resistance alleles present in different wheat genotypes. In order to understand the functional importance of the amino acid polymorphisms between the active immune receptor PM3A and the inactive ancestral variant PM3CS, we exchanged polymorphic regions and residues in the LRR domain of PM3A with the corresponding segments of PM3CS. These novel variants were functionally tested for recognition of the corresponding AVRPM3<sup>A2/F2</sup> avirulence protein in Nicotiana benthamiana. We identified polymorphic residues in four regions of PM3A that enhance the immune response, but also residues that reduce it or result in complete loss of function. We found that the identified critical residues in PM3A modify its activation threshold towards different protein variants of AVRPM3<sup>A2/F2</sup> . PM3A variants with a lowered threshold gave a stronger overall response and gained an extended recognition spectrum. One of these variant proteins with a single amino acid change was stably transformed into wheat where it conferred race-specific resistance to mildew. This is a proof of concept that improved PM3A variants with an enlarged recognition spectrum can be engineered based on natural diversity by exchanging single or multiple residues that modulate resistance function.</AbstractText><CopyrightInformation>This article is protected by copyright. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lindner</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-3142-9013</Identifier><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Keller</LastName><ForeName>Bettina</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pal Singh</LastName><ForeName>Simrat</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hasenkamp</LastName><ForeName>Zsuzsanna</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jung</LastName><ForeName>Esther</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>Marion C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bourras</LastName><ForeName>Salim</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Keller</LastName><ForeName>Beat</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Triticum aestivum
</Keyword><Keyword MajorTopicYN="N">Blumeria graminis f.sp. tritici</Keyword><Keyword MajorTopicYN="N">NLR-type receptor</Keyword><Keyword MajorTopicYN="N">allelic diversity</Keyword><Keyword MajorTopicYN="N">genetic engineering</Keyword><Keyword MajorTopicYN="N">mutagenesis</Keyword><Keyword MajorTopicYN="N">plant immunity</Keyword><Keyword MajorTopicYN="N">resistance gene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32645755</ArticleId><ArticleId IdType="doi">10.1111/tpj.14917</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suisse</li><li>Suède</li></country><region><li>Canton de Zurich</li></region><settlement><li>Zurich</li></settlement><orgName><li>Université de Zurich</li></orgName></list><tree><country name="Suisse"><region name="Canton de Zurich"><name sortKey="Lindner, Stefan" sort="Lindner, Stefan" uniqKey="Lindner S" first="Stefan" last="Lindner">Stefan Lindner</name></region><name sortKey="Bourras, Salim" sort="Bourras, Salim" uniqKey="Bourras S" first="Salim" last="Bourras">Salim Bourras</name><name sortKey="Hasenkamp, Zsuzsanna" sort="Hasenkamp, Zsuzsanna" uniqKey="Hasenkamp Z" first="Zsuzsanna" last="Hasenkamp">Zsuzsanna Hasenkamp</name><name sortKey="Jung, Esther" sort="Jung, Esther" uniqKey="Jung E" first="Esther" last="Jung">Esther Jung</name><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><name sortKey="Keller, Bettina" sort="Keller, Bettina" uniqKey="Keller B" first="Bettina" last="Keller">Bettina Keller</name><name sortKey="Muller, Marion C" sort="Muller, Marion C" uniqKey="Muller M" first="Marion C" last="Müller">Marion C. Müller</name><name sortKey="Pal Singh, Simrat" sort="Pal Singh, Simrat" uniqKey="Pal Singh S" first="Simrat" last="Pal Singh">Simrat Pal Singh</name></country><country name="Suède"><noRegion><name sortKey="Bourras, Salim" sort="Bourras, Salim" uniqKey="Bourras S" first="Salim" last="Bourras">Salim Bourras</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantPathoEffV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000051 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000051 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PlantPathoEffV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32645755
|texte= Single residues in the LRR domain of the wheat PM3A immune receptor can control the strength and the spectrum of the immune response.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32645755" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PlantPathoEffV1
| This area was generated with Dilib version V0.6.38. |  |